In order to detect the titers of the T.denticola, P. gingivalis, and B.forsythus, which can be considered to be periodontopathic, possess a trypsin-like enzyme that can be detected by the hydrolysis of N-benzoyl-DL-arginine-2-naphthylamide(BANA),
45
patients(29 patients(29 men, 16 women, average age 44.27) with adult periodontitis were selected.
Pocket depth, gingival crevicular fluid and trypsin-like activity were measured on the buccal surface of upper right and left first molars molars, lingual surface of lower right and left first molars, labial surface of upper right central incisor
and
labial surface of upper left central incisor by simplified oral hygiene index.
The amount of gingival crevicual fluid flow was measured by PeriotronR(Harco-600, Canada), and periodontal pocket depth was measured by Michgan-O probe. And then trypsin-like activity was evaluated using PerioScan reagent cardR(Oral-B
Laboratories,
Redwood City, CA).
Trypsin-like acitivity to the amount of gingival crevicular fluid flow was statistically analysed by percentage, and the change between BANA hydrolysis reaction and the amount of gingival crevicular fluid flow and pocket depth was statistically
analysed
by SAS using ANOVA, respectively.
@ES The results were as follows:
@EN 1. The amount of gingival crevicular fluid flow was chiefly BNAN-negative(12.40%) in 20-29 group, BNAN-weakly positive(8.26%) in 30-30 group, and chiefly BNAN-positive (11.16%) in 50 or more group.
2. The change between BANA hydrolysis reaction and the amount of gignival crevicular fluid flow and pocket depth was statistically significant (P<0.05)
|